The MultiBac platform

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The MultiBac platform at the EMBL is the lead technology for eukaryotic multiprotein complex production [Berger et al, Nat. Biotechnol. 2004; Fitzgerald et al, Nat. Methods 2006; Bieniossek et al, TiBS 2012]. MultiBac is an advanced baculovirus expression vector system (BEVS) particularly tailored for protein complex production. Since its introduction it has been distributed to more than 400 laboratories (academic and industrial) worldwide and has catalysed research in many areas of the life sciences [see, for example, Imasaki et al, Nature 2011; Yamada et al, Nature 2011; Schreiber et al, Nature 2011; Leonard et al, Cell 2011; Zeqiraj et al, Science 2009].

MultiBac is installed at the Eukaryotic Expression Facility (EEF) in the Berger laboratory at EMBL Grenoble. The EEF consists of a fully equipped cell culture facility including sterile benches, light microscopy, fluorescence microscopy, shaker platforms, incubators and tissue culture equipment, up-stream (USP) and down-stream processing (DSP) equipment and facilities for protein purification and analysis including state-of-the-art  biophysical techniques.
MultiBac utilizes an optimized baculovirus that has been engineered in the Berger lab for high-quality protein production. A fluorescent marker has been integrated into the viral backbone to enable virus performance tracking and production of complex biologics. This virus is accessed by multigene constructs containing the DNA encoding for the protein complex produced. This multigene construct is generated from small synthetic precursor plasmid modules into which the genes of interest are inserted. Interfaced with the proprietary ACEMBL pipeline the MultiBac platform can be used for biologics production in high-throughput mode.

MultiBac was awarded prestigious prizes including the Swiss Technology Award in 2007, and continues to attract competitive funding from the European Commission since FP6 and throughout FP7. The MultiBac platform has been accessed by many scientists from Europe and world-wide for intensive training and for protein production. It is successfully integrated in numerous co-developments, R&D and discovery programs, in academia and industry.